Precision will be reduced, but yield will be increased Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following is a codominant marker?

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#Question id: 16025

#Section 6: Plant, Animal and Microbial Biotechnology

The technique of organ culture may be divided on the basis of employing

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#Question id: 30

#Section 2: General Biology

Amongst the following chemical elements that are essential to organisms, which of the following set of elements have an atomic number above that of selenium?

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#Question id: 1081

#Section 3: Genetics, Cellular and Molecular Biology

Which of the following is a type of local signaling in which a cell secretes a signal molecule that affects neighboring cells?

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#Question id: 338

#Section 2: General Biology

What is the difference between covalent bonds and ionic bonds?

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#Question id: 14175

#Section 5: Bioprocess Engineering and Process Biotechnology

The growth of S. cerevisiae on glucose under anaerobic conditions can be described by the following overall reaction

Determine the biomass yield coefficient YX/S?