Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following techniques doesn’t involve use of a secondary antibody?

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#Question id: 16569

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Eukaryotic mRNA extraction, can be done by using which of the following columns in affinity chromatography 

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#Question id: 16570

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Agarose gels are used for the electrophoresis of

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#Question id: 16571

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Polyacrylamide gels are formed from the polymerization of acrylamide monomer in the presence of

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#Question id: 16572

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

During the casting of polyacrylamide gel acrylamide and bis-acrylamide are joined by 

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#Question id: 16573

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Which the following statements is/are true about the stacking gel?

TLS Online TPP Program

#Question id: 16574

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

How many different types of dNTPs are utilized in a reaction mixture when carrying out sanger’s DNA sequencing