Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following techniques doesn’t involve use of a secondary antibody?

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#Question id: 11804

#Section 6: Plant, Animal and Microbial Biotechnology

P-proteins have been characterized at the molecular level. P-proteins from the genus Cucurbita consist of two major proteins;

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#Question id: 14400

#Section 5: Bioprocess Engineering and Process Biotechnology

internal mass transfer effects are eliminated when the internal effective ness factor is 

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#Question id: 14775

#Section 1: Engineering Mathematics

A bag contains 3 black and 5 white balls. One ball is drawn from the bag. What is the probability that the ball is not black ?

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#Question id: 3541

#Section 3: Genetics, Cellular and Molecular Biology

Blue follower is dominant over white flower and long pollen is dominant over short pollen. A plant with Blue flower and short pollen was cross with white flower and long pollen. All F1 offspring display blue flower and long pollen. What is the percent of F2 progeny showing blue flower and long pollen?

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#Question id: 3686

#Section 3: Genetics, Cellular and Molecular Biology

The short strand of ........ primer is required for the replication of DNA: