Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the statement is worng? Aminopetrin………s

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#Question id: 3786

#Section 3: Genetics, Cellular and Molecular Biology

E. coli cells grown on 15N medium are transferred to 14N medium and allowed to grow for two more generations (two rounds of DNA replication). DNA extracted from these cells is centrifuged. What density distribution of DNA would you expect in this experiment?

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#Question id: 14492

#Section 1: Engineering Mathematics

A die is rolled. The probability of getting a number 1 or 6 on the upper face is

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#Question id: 1701

#Section 2: General Biology

Which of the following statements is TRUE of superantigens?

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#Question id: 549

#Section 2: General Biology

Which one of the following statements is true of enzyme catalysts?

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#Question id: 14493

#Section 1: Engineering Mathematics

The probability of the horse A winning the race is 1/5 and the probability of the horse B winning the same race is 1/6, then the probability of one of the horse to win the race is