Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

A restriction enzyme cleaves a circular plasmid vector to produce fragments : 1kb, 0.3kb, 2kb, 1kb and 0.3kb. How many bands will be visible on agarose gel?

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#Question id: 4752

#Section 3: Genetics, Cellular and Molecular Biology

Red-green color blindness is caused by a sex-linked recessive allele. A color-blind man marries a woman with normal vision whose father was color-blind. What is the probability that they will have a color-blind daughter? What is the probability that their first son will be color-blind respectively?

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#Question id: 9583

#Section 2: General Biology

 A mechanism known as the Q cycle accounts for most of the observations in which structure and reactions of plastoquinone that operate in,

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#Question id: 3908

#Section 3: Genetics, Cellular and Molecular Biology

Which of the following is correct?

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#Question id: 14139

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Which of the following statement is incorrect about sequence similarity and sequence identity? 

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#Question id: 21379

#Section 5: Bioprocess Engineering and Process Biotechnology

A balanced chemical reaction equation conforms to the law of