Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Arrange in the order of their increasing capacity:

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#Question id: 27362

#Section 5: Bioprocess Engineering and Process Biotechnology

Continuous stirred tank fermenter is characterized by (biomass concentration X kg/m3, residual substrate is S kg/m3, Sf=substrate concentration in feed kg/m3, Dilution rate = D and µm>>D and Km<Sf, XD= the biomass productivity kg/m3h. If the dilution rate is doubled to 2D then the new steady state values in comparison with earlier steady state is characterized by

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#Question id: 27363

#Section 3: Genetics, Cellular and Molecular Biology

Match the types of RNA in Group I with their corresponding function in Group II

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#Question id: 27364

#Section 3: Genetics, Cellular and Molecular Biology

Which statement best describes the distinction between the purposes of the two RNAi pathways within the eukaryotic cell?

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#Question id: 27365

#Section 3: Genetics, Cellular and Molecular Biology

During siRNA-mediated gene silencing within the homo sapiens cell, the RNA-induced Silencing Complex (RISC) is composed of which of the following protein arrangements?

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#Question id: 27366

#Section 3: Genetics, Cellular and Molecular Biology

If the siRNA is a complete homolog of the target mRNA sequences what is the net result?

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#Question id: 27367

#Section 3: Genetics, Cellular and Molecular Biology

The miRNAs are transcribed from non-protein encoding genes and are typically __________ nucleotides long.