Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

 Which of the following is true for traditional and real time PCR?

#Question id: 3797

#Section 3: Genetics, Cellular and Molecular Biology

Throughout interphase, _______ are synthesized at a relatively constant rate, whereas _______ are synthesized only during the S phase.

#Question id: 3584

#Section 2: General Biology

In a plant species, a segregating line (RR- Red, Rr- Pink and rr- white) that contains both homozygotes and heterozygotes at a locus can be made homozygous by repeated selfing for several generations. What is the proportional red flower in third generations of selfing, if the level of heterozygosity in generation '0' is denoted as 1?

#Question id: 2103

#Section 2: General Biology

When a plant cell, such as one from a tulip leaf, is submerged in a hypertonic solution, what is likely to occur?

#Question id: 14859

#Section 4: Fundamentals of Biological Engineering

#Question id: 14288

#Section 5: Bioprocess Engineering and Process Biotechnology

During a batch culture experiment the following data was obtained 

What will be the product yield (g) per gram of substrates?