Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

 Which of the following is true for traditional and real time PCR?

#Question id: 9281

#Section 1: Engineering Mathematics

#Question id: 9282

#Section 2: General Biology

The rate of photosynthesis when red and far-red light are given together is greater than the sum of the rates when they are given apart, the effect provided essential

evidence in favor of the concept that photosynthesis is carried out by two photochemical systems working in tandem but with slightly different wavelength optima

this effect is known as

#Question id: 9284

#Section 2: General Biology

Following are some facts regarding spatial separation of PSI and PSII. Which one would be INCORRECT?

#Question id: 9285

#Section 2: General Biology

#Question id: 9581

#Section 2: General Biology

These four Photosystem II, Cytochrome b6 f, Photosystem I and ATP synthase integral membrane complexes are vectorially oriented in the thylakoid membrane to function as follows, find out the incorrect statements;

#Question id: 9582

#Section 2: General Biology

Electrons ejected from chlorophyll travel through a series of electron carriers organized in the “Z Scheme” shows a current version of the Z scheme, in which all the electron carriers known to function in electron flow from H2O to NADP Presented in the following sequence;

a.) P680 reduce by Yz has received electrons from oxidation of water P680→P680*→pheophytin→ plastoquinones→ cytochrome b6 f complex→ plastocyanin→ in turn reduces (PSI) P700+

b.) P700+→ P700*→ membrane-bound iron–sulfur proteins → A0→ A1 → flavoprotein ferredoxin–NADP reductase (FNR) soluble → ferredoxin(Fd) → NADP+ to NADPH

c.) P700+→ P700*→ A0→ A1→ membrane-bound iron–sulfur proteins→ ferredoxin(Fd)→ soluble flavoprotein ferredoxin–NADP reductase (FNR)→ NADP+ to NADPH

d.) P680 reduce by Yz has received electrons from oxidation of water P680→P680*→ plastoquinones → pheophytin → cytochrome b6 f complex→ plastocyanin→ in turn reduces (PSI) P700+

Which one of the following combinations is correct?