Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

What would the expected effect be on a PCR reaction if the primers used were slightly shorter and more variable than the intended oligonucleotide sequences?

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#Question id: 14802

#Section 5: Bioprocess Engineering and Process Biotechnology

Column I                                                                                  column II

A. Hydrophobic interaction chromatography (HIC)          1. gradient stepwise

B. Ion exchange chromatography                                       2. isocratic

C. Gel filtration chromatography                                         3. stepwise

D. Affinity chromatography                                                  4.  Gradient stepwise

A       B       C      D

1        2       3       4

3        1       2       4

1       4        2       3

1       3        4       2

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#Question id: 3644

#Section 3: Genetics, Cellular and Molecular Biology

In replication: soon after synthesis, the DNA fragments are joined to make one continuous piece of DNA by the enzyme:

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#Question id: 10140

#Section 2: General Biology

Following are certain statements regarding activated and inactivated form of Rubisco, Which one is INCORRECT?

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#Question id: 1128

#Section 3: Genetics, Cellular and Molecular Biology

During the epinephrine stimulated conversion of glycogen to glucose 1-phosphate, which of the following steps involves protein phosphorylation?

a. inactivation of glycogen synthase               b. activation of protein kinase A

c. activation of adenylyl cyclise                      d. activation of glycogen phosphorylase

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#Question id: 15485

#Section 6: Plant, Animal and Microbial Biotechnology

Plasmid vector systems for cloning in which bacteria have been established, for amino acid production and related strains.

A. Corynebacterium

B. Micrococcus, 

C. Brevibacterium, 

D. Microbacterium

Choose the correct option.