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TLS Online TPP Program

#Question id: 13039

 Which of the following is incorrect about a microarray?

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology
  1. It is a slide attached with a high-density array of immobilized DNA oligomers representing the entire genome of the species under study 
  2. Array of immobilized DNA oligomers cannot be cDNAs
  3. Each oligomer is spotted on the slide and serves as a probe for binding to a unique complementary cDNA 
  4. It is the most commonly used global gene expression profiling method
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TLS Online TPP Program

#Question id: 14261

#Section 5: Bioprocess Engineering and Process Biotechnology

C. roseus cells immobilized in Ca-alginate beads of diameter 0.5 mm are used for production of indole alkaloids (IA) in a fluidized-bed bioreactor. The rate limiting nutrient is glucose and no intraparticle diffusion limitations exist. Use the following data: Flow rate of the feed: Q = 1 l/h, Glucose in the feed: So = 30 g/l, Plant Cell Concentration: X = 6 g/l reac. The rate constant for IA formation: k = 5 d-1 (g/l)^-1 Ks = 0.4 g/l, Column diameter: Do = 0.15 m. Growth is negligible and Monod kinetics is valid. If Yp/s is 0.02 g IA/g glu, determine IA concentration in the effluent.
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TLS Online TPP Program

#Question id: 14262

#Section 5: Bioprocess Engineering and Process Biotechnology

The BOD5 value of a waste-water feed stream to an activated-sludge unit is S0 = 300 mg/l, and the effluent is desired to be S = 30 mg/l. The feed flow rate is F = 2× 10^7 l/day. For the recycle ratio of α = 0.5 and a steady-state biomass concentration of X = 5 g/l, Use the following kinetic parameters:

 
calculate Required reactor volume (V). 
_______________10^6
TLS Online TPP Program

#Question id: 14263

#Section 5: Bioprocess Engineering and Process Biotechnology

The BOD5 value of a waste-water feed stream to an activated-sludge unit is S0 = 300 mg/l, and the effluent is desired to be S = 30 mg/l. The feed flow rate is F = 2× 10^7 l/day. For the recycle ratio of α = 0.5 and a steady-state biomass concentration of X = 5 g/l, Use the following kinetic parameters:

 
calculate Biomass concentration in recycle (Xr).
TLS Online TPP Program

#Question id: 14264

#Section 5: Bioprocess Engineering and Process Biotechnology

The BOD5 value of a waste-water feed stream to an activated-sludge unit is S0 = 300 mg/l, and the effluent is desired to be S = 30 mg/l. The feed flow rate is F = 2× 10^7 l/day. For the recycle ratio of α = 0.5 and a steady-state biomass concentration of X = 5 g/l, Use the following kinetic parameters:

 
calculate Hydraulic residence time
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TLS Online TPP Program

#Question id: 14265

#Section 5: Bioprocess Engineering and Process Biotechnology

For the activated-sludge, the specific growth rate of cells is given by 


 

The following parameter values are known: F = 500 l/h, α = 0.4, ɣ = 0.1, Xe = 0, V = 1500 l, Ks = 10 mg/l, µm = 1 h-1 , kd = 0.05 h-1 , S0 = 1000 mg/l, Y M X/S = 0.5 g dw/g substrate. Calculate the substrate concentration (S) in the reactor at steady state. -------------------------
TLS Online TPP Program

#Question id: 14266

#Section 5: Bioprocess Engineering and Process Biotechnology

For the activated-sludge, the specific growth rate of cells is given by 


 

The following parameter values are known: F = 500 l/h, α = 0.4, ɣ = 0.1, Xe = 0, V = 1500 l, Ks = 10 mg/l, µm = 1 h-1 , kd = 0.05 h^-1 , S0 = 1000 mg/l, Y M X/S = 0.5 g dw/g substrate.. Calculate the cell concentration in the reactor.