TLS Online TPP Program

#Question id: 13037


Which of the following proteins will be most far away from the positively charged electrode during isoelectric focusing in a buffer with pH of 5?

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology
  1. Protein A with IP of 8
  2. Protein B with IP of 4
  3. Protein C with IP of 7
  4. Protein D with IP of 3
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TLS Online TPP Program

#Question id: 14334

#Section 5: Bioprocess Engineering and Process Biotechnology

Laboratory-scale fermenters are usually mixed using small stirrers with electric motors rated between 100 and 500 W. One such motor is used to drive a 7cm turbine impeller in a small reactor containing fluid with the properties of water. The stirrer speed is 900 rpm. Estimate the power requirements for this process.

TLS Online TPP Program

#Question id: 14336

#Section 5: Bioprocess Engineering and Process Biotechnology

A cylindrical stirred bioreactor of diameter and height 2 m has a Rushton turbine one-third the tank diameter in size. The bioreactor contains Newtonian culture broth with the same density as water and with viscosity 4 cP. The tank is now aerated. In the presence of gas bubbles, the approximate relationship between ungassed power number (NP)0 and gassed power number (NP)g is: (NP)g =0.5 (NP)0. What maximum stirrer speed is now possible in the sparged reactor? Estimate the mixing time.

TLS Online TPP Program

#Question id: 14337

#Section 5: Bioprocess Engineering and Process Biotechnology

A fermentation broth with viscosity 10 −2 Pa s and density 1000 kg m − 3 is agitated in a 2.7 m^ 3 baffled tank using a Rushton turbine with diameter 0.5 m and stirred speed 1 s ^−1. Estimate the mixing time.

TLS Online TPP Program

#Question id: 14338

#Section 5: Bioprocess Engineering and Process Biotechnology

Quasi steady state condition is 

TLS Online TPP Program

#Question id: 14339

#Section 5: Bioprocess Engineering and Process Biotechnology

In continuous culture which phase is usually longer?