In order to quantify unknown DNA templates, a standard dilution is prepared using DNA of known concentration. As the DNA accumulates during the early exponential phase of the reaction,an arbitrary point is taken where each of the diluted DNA samples cross. This is termed as_____

TLS Online TPP Program

#Question id: 14336

#Section 5: Bioprocess Engineering and Process Biotechnology

A cylindrical stirred bioreactor of diameter and height 2 m has a Rushton turbine one-third the tank diameter in size. The bioreactor contains Newtonian culture broth with the same density as water and with viscosity 4 cP. The tank is now aerated. In the presence of gas bubbles, the approximate relationship between ungassed power number (N_P)₀ and gassed power number (N_P)g is: (N_P)g =0.5 (N_P)₀. What maximum stirrer speed is now possible in the sparged reactor? Estimate the mixing time.

TLS Online TPP Program

#Question id: 14337

#Section 5: Bioprocess Engineering and Process Biotechnology

A fermentation broth with viscosity 10 −2 Pa s and density 1000 kg m − 3 is agitated in a 2.7 m^ 3 baffled tank using a Rushton turbine with diameter 0.5 m and stirred speed 1 s ^−1. Estimate the mixing time.

TLS Online TPP Program

#Question id: 14338

#Section 5: Bioprocess Engineering and Process Biotechnology

Quasi steady state condition is 

TLS Online TPP Program

#Question id: 14339

#Section 5: Bioprocess Engineering and Process Biotechnology

In continuous culture which phase is usually longer? 

TLS Online TPP Program

#Question id: 14340

#Section 5: Bioprocess Engineering and Process Biotechnology

Which bioreactor consists of a multi-layered bag instead of vessel