According to the diagram, arrange the steps in correct order: 

1. DNA is then digested by the Cas9 nuclease at specific points on both DNA strands. 

2. Homologous recombination to repair the break and incorporate the new DNA sequence then follows. 

3.The nuclease Cas9 is targeted to DNA by a guide RNA consisting of a specific 20 nucleotide spacer/targeting sequence (crRNA).

 4. That identifies the DNA to be modified, linked to a trans-activating (tracrRNA) sequence necessary for the recruitment and stability of Cas9 nuclease.

#Question id: 9285

#Section 2: General Biology

#Question id: 9581

#Section 2: General Biology

These four Photosystem II, Cytochrome b6 f, Photosystem I and ATP synthase integral membrane complexes are vectorially oriented in the thylakoid membrane to function as follows, find out the incorrect statements;

#Question id: 9582

#Section 2: General Biology

Electrons ejected from chlorophyll travel through a series of electron carriers organized in the “Z Scheme” shows a current version of the Z scheme, in which all the electron carriers known to function in electron flow from H2O to NADP Presented in the following sequence;

a.) P680 reduce by Yz has received electrons from oxidation of water P680→P680*→pheophytin→ plastoquinones→ cytochrome b6 f complex→ plastocyanin→ in turn reduces (PSI) P700+

b.) P700+→ P700*→ membrane-bound iron–sulfur proteins → A0→ A1 → flavoprotein ferredoxin–NADP reductase (FNR) soluble → ferredoxin(Fd) → NADP+ to NADPH

c.) P700+→ P700*→ A0→ A1→ membrane-bound iron–sulfur proteins→ ferredoxin(Fd)→ soluble flavoprotein ferredoxin–NADP reductase (FNR)→ NADP+ to NADPH

d.) P680 reduce by Yz has received electrons from oxidation of water P680→P680*→ plastoquinones → pheophytin → cytochrome b6 f complex→ plastocyanin→ in turn reduces (PSI) P700+

Which one of the following combinations is correct?

#Question id: 9583

#Section 2: General Biology

 A mechanism known as the Q cycle accounts for most of the observations in which structure and reactions of plastoquinone that operate in,

#Question id: 9585

#Section 2: General Biology

In light reaction the reaction centre of PSII  receives electron from water molecules by splitting into oxygen, If 5 oxygen molecules evolved per 50 quanta was

absorbed then what is the quantum yield?

#Question id: 9586

#Section 2: General Biology

The photochemical quantum efficiency (quantum yield) is nearly 100%, the energy conversion efficiency is only 27%, and the overall efficiency of conversion of solar

energy is only a few percent the solar energy storage efficiency is a measure of how much of the energy in the entire solar spectrum is converted to usable form, if it

can be measure in terms of C3 and C4 plant?