TLS Online TPP Program

#Question id: 10524


Members of the Poaceae family, which includes all of the cereal crops, use benzoxazinoids, these alkaloids shows;

a) Alkaloids are derived from the tryptophan as constitutively produced defensive secondary metabolites

b) These benzoxazinoids such as DIBOA and its derivative DIMBOA are usually stored in the peroxisome

c) Upon damage, the inactive glycosides are hydrolyzed, generating glucosinolate, which are toxic not only to insect herbivores but also against pathogens

d) DIBOA and DIMBOA perform insecticidal and pesticidal activities

e) It can be inactivated by hydroxylation and N-glycosylation reactions

Which of the following combination is correct about benzoxazinoids alkaloids?

#Section 6: Plant, Animal and Microbial Biotechnology
  1. B and C  
  2. A, C and D 
  3. B, C and E 
  4. A, D and E
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#Question id: 13064

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
Which of the following techniques doesn’t involve use of a secondary antibody?