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TLS Online TPP Program

#Question id: 381

Consider an acetate buffer, initially at the same pH as its pKa (4.76). When sodium hydroxide (NaOH) is mixed with this buffer, the:

#Section 4: Fundamentals of Biological Engineering

  1. pH rises more than if an equal amount of NaOH is added to an acetate buffer initially at pH 6.76

  2. pH rises more than if an equal amount of NaOH is added to unbuffered water at pH 4.76.

  3. ratio of acetic acid to sodium acetate in the buffer falls.

  4. sodium acetate formed precipitates because it is less soluble than acetic acid.

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TLS Online TPP Program

#Question id: 13071

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Modifications of the agglutination reaction involve the use of ________particles, which allow the reaction to be observed more easily in the liquid phase
TLS Online TPP Program

#Question id: 13072

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Inheritance pattern of RFLP and RAPD markers for genome mapping in plants;
TLS Online TPP Program

#Question id: 13073

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Restriction enzymes must be use in RFLP and RAPD markers for genome mapping in plants;
TLS Online TPP Program

#Question id: 13074

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Type of probe used in RFLP;
TLS Online TPP Program

#Question id: 13075

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Radioisotopes must be used in RFLP and RAPD markers
TLS Online TPP Program

#Question id: 13076

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Restriction fragment length polymorphism denotes that a single restriction enzyme produces fragments of different lengths from the same stretch of genomic DNA of different strains of a species or from different related species. RFLPs are detected as follows:

i.  Large molecular weight genomic DNA is isolated from several strains or related species;

ii.  The fragments in these digests are separated through electrophoresis

iii.  These 'DNAs are then digested with a selected restriction enzyme

iv.  Exposed to a suitably radio-labelled appropriate DNA probe under conditions favouring DNA: DNA hybridization

v.  The resulting gel lanes are transferred and fixed to a suitable solid support and

vi.   The free probes are removed

vii.  The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.