TLS Online TPP Program

#Question id: 383


Three buffers are made by combining a 1 M solution of acetic acid with a 1 M solution of sodium acetate in the ratios shown below

1 M acetic acid              

1 M sodium acetate  

Buffer 1: 10 mL

 90 mL

Buffer 2: 50 mL

50 mL

Buffer 3: 90 mL

10 mL

 Which of these statements is true of the resulting buffers?

#Section 4: Fundamentals of Biological Engineering
  1. pH of buffer 1 < pH of buffer 2 < pH of buffer 3

  2. pH of buffer 1 = pH of buffer 2 = pH of buffer 3

  3. pH of buffer 1 > pH of buffer 2 > pH of buffer 3

  4. The problem cannot be solved without knowing the value of pKa.

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TLS Online TPP Program

#Question id: 27354

#Section 3: Genetics, Cellular and Molecular Biology

What does the cas9 protein do?

TLS Online TPP Program

#Question id: 27355

#Section 3: Genetics, Cellular and Molecular Biology

How does the guide RNA help the cell know where to cut the DNA?

TLS Online TPP Program

#Question id: 27356

#Section 3: Genetics, Cellular and Molecular Biology

What is the role of the guide RNA?

TLS Online TPP Program

#Question id: 27359

#Section 5: Bioprocess Engineering and Process Biotechnology

A double reciprocal plot was created from the specific growth rate and limiting-substrate concentration data obtained from a chemostat experiment. A linear regression gave values of 1.25 h and 100 mg hL-1 for the intercept and slope, respectively.
(a) The respective values of the Monod kinetic constants μm (h-1) and K. (mg L-¹) are as follows:

TLS Online TPP Program

#Question id: 27360

#Section 5: Bioprocess Engineering and Process Biotechnology

The same culture (with the m and K, values as computed above) is cultivated in a 10 L chemostat being operated with a 50 mL min-1 sterile feed containing 50 g L-1 of substrate. Assuming an overall yield coefficient of 0.3 (g- DCW) (g- substrate), the respective values of the outlet biomass and substrate concentrations are

TLS Online TPP Program

#Question id: 27361

#Section 5: Bioprocess Engineering and Process Biotechnology

A continuous stirred tank bioreactor produces 48 kg lysine.day-1. If the volumetric productivity is 2 g.l-1.h-1, the volume of the reactor is: