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TLS Online TPP Program

#Question id: 397

The loss of water from a plant by transpiration cools the leaf. Movement of water in transpiration requires both adhesion to the conducting walls and wood fibers of the plant and cohesion of the molecules to each other. A scientist wanted to increase the rate of transpiration of a crop species to extend its range into warmer climates. The scientist substituted a nonpolar solution with an atomic mass similar to that of water for hydrating the plants. What do you expect the scientist's data will indicate from this experiment?

#Section 4: Fundamentals of Biological Engineering

  1. The rate of transpiration will be the same for both water and the nonpolar substance.

  2. The rate of transpiration will be slightly lower with the nonpolar substance as the plant will not have evolved with the nonpolar compound.

  3. Transpiration rates will fall to zero as nonpolar compounds do not have the properties necessary for adhesion and cohesion.

  4. Transpiration rates will increase as nonpolar compounds undergo adhesion and cohesion with wood fibers more readily than water.

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TLS Online TPP Program

#Question id: 14622

#Section 2: General Biology

Endospores are
TLS Online TPP Program

#Question id: 14623

#Section 5: Bioprocess Engineering and Process Biotechnology

a chromatographic separation of a two component samples on 50 cm column gave the retention times for the solutes A and B as 2.5 and 3.1 minutes with base widths of the two chromatographic peaks being 0.24 and 0.3 min respectively. Calculate resolution of two peak 
TLS Online TPP Program

#Question id: 14624

#Section 5: Bioprocess Engineering and Process Biotechnology

An enzyme is produced for manufacturing a sun protection lotion. Given kinetic data for the enzyme reaction with vmax= 2.5mmol M-3 S-1 Km= 8.9 mM and So = 12 mM, what would be the time required for 95% conversion in a batch bioreactor? 
TLS Online TPP Program

#Question id: 14625

#Section 5: Bioprocess Engineering and Process Biotechnology

Monod kinetics are considered in a CSTR with an organism growing with an initial substrate concentration of 50 g1-1 and kinetic parameters of Ks = 2 g1-1 and umax = 0.5 h-1 . (a) What would be the maximum dilution rate for 100% yield of biomass with maximum rate? 
TLS Online TPP Program

#Question id: 14626

#Section 5: Bioprocess Engineering and Process Biotechnology

A bacterial fermentation was carried out in a reactor containing broth with average density 1200 kg/m3 and viscosity 0.02 N.s/m2 . The broth was agitated at 90 rpm and air was introduced through the sparger at a flow rate of 0.4 vvm. The fermenter was equipped with two sets of flat blade turbine impellers and four baffles. The dimensions of vessel, impellers and baffle width were: tank diameter, Dt =4 m; impeller diameter, Di = 2 m; baffle width, Wb = 0.4 m; also, the liquid depth was H = 6.5 m 
Determine ungassed power, P; 
TLS Online TPP Program

#Question id: 14627

#Section 5: Bioprocess Engineering and Process Biotechnology

In a batch production of penicillin, 40 m3 capacity of the plant, sterile air is required to be supplied. The bioreactor requires 1 vvm (volume of air/volume of broth. min). The incoming air contains 3000 cells/m3of air, for 100 hours operation. Calculate the depth of filter if the penetration of bacteria is 1 in 1 million.