TLS Online TPP Program

#Question id: 397


The loss of water from a plant by transpiration cools the leaf. Movement of water in transpiration requires both adhesion to the conducting walls and wood fibers of the plant and cohesion of the molecules to each other. A scientist wanted to increase the rate of transpiration of a crop species to extend its range into warmer climates. The scientist substituted a nonpolar solution with an atomic mass similar to that of water for hydrating the plants. What do you expect the scientist's data will indicate from this experiment?

#Section 4: Fundamentals of Biological Engineering
  1. The rate of transpiration will be the same for both water and the nonpolar substance.

  2. The rate of transpiration will be slightly lower with the nonpolar substance as the plant will not have evolved with the nonpolar compound.

  3. Transpiration rates will fall to zero as nonpolar compounds do not have the properties necessary for adhesion and cohesion.

  4. Transpiration rates will increase as nonpolar compounds undergo adhesion and cohesion with wood fibers more readily than water.

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TLS Online TPP Program

#Question id: 14190

#Section 5: Bioprocess Engineering and Process Biotechnology

Aerobic degradation of an organic compound by a mixed culture of organisms in waste water can be represented by the following reaction

               

Determine RQ for the organisms

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TLS Online TPP Program

#Question id: 14191

#Section 5: Bioprocess Engineering and Process Biotechnology

Consider a 1000-l CSTR in which biomass is being produced with glucose as the substrate. The microbial system follows a Monod relationship withn ยตm = 0.4 h-1 , KS = 1.5 g/l (an unusu ally high value), and the yield factor YX/S = 0.5 g biomass/g substrate consumed. If normal operation is with a sterile feed containing 10 g/l glucose at a rate of 100 l/h. What is the specific biomass production rate (g/l-h) at steady state?

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TLS Online TPP Program

#Question id: 14192

#Section 5: Bioprocess Engineering and Process Biotechnology

In a two-stage chemostat system, the volumes of the first and second reactors are V1 = 500 l and V2 = 300 l, respectively. The first reactor is used for biomass production and the second is for a secondary metabolite formation. The feed flow rate to the first reactor is F = 100 l/h, and the glucose concentration in the feed is S = 5.0 g/l. Use the following constants for the cells.



Determine cell concentration in the effluent of the first stage.

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TLS Online TPP Program

#Question id: 14193

#Section 5: Bioprocess Engineering and Process Biotechnology

In a two-stage chemostat system, the volumes of the first and second reactors are V1 = 500 l and V2 = 300 l, respectively. The first reactor is used for biomass production and the second is for a secondary metabolite formation. The feed flow rate to the first reactor is F = 100 l/h, and the glucose concentration in the feed is S = 5.0 g/l. Use the following constants for the cells.



Determine glucose concentration in the effluent of the first stage. _____________________

TLS Online TPP Program

#Question id: 14194

#Section 5: Bioprocess Engineering and Process Biotechnology

Penicillin is produced by P. chrysogenum in a fed-batch culture with the intermittent addition of glucose solution to the culture medium. The initial culture volume at quasi-steady state is V0 = 500 l, and glucose-containing nutrient solution is added with a flow rate of F = 50 l/h. Glucose concentration in the feed solution and initial cell concentration are S0 = 300 g/l and X0 = 20 g/l, respectively. The kinetic and yield coefficients of the organism are mm = 0.2 h-1 , KS = 0.5 g/l, and YX/S = 0.3 g dw/g glucose. Determine the culture volume at t = 10 h

TLS Online TPP Program

#Question id: 14195

#Section 5: Bioprocess Engineering and Process Biotechnology

Penicillin is produced by P. chrysogenum in a fed-batch culture with the intermittent addition of glucose solution to the culture medium. The initial culture volume at quasi-steady state is V0 = 500 l, and glucose-containing nutrient solution is added with a flow rate of F = 50 l/h. Glucose concentration in the feed solution and initial cell concentration are S0 = 300 g/l and X0 = 20 g/l, respectively. The kinetic and yield coefficients of the organism are mm = 0.2 h-1 , KS = 0.5 g/l, and YX/S = 0.3 g dw/g glucose. Determine the concentration of glucose at t = 10 h at quasi-steady state