Following are certain statements regarding furanocoumarins;

 a) furanocoumarins have an attached furan ring

 b) These compounds are toxic until they are activated by sunlight in the ultraviolet A (UV-A) region (320–400 nm)  

 c) Light activated furanocoumarins can insert themselves into the double helix of DNA and bind to the pyrimidine bases, thus blocking transcription and repair and leading eventually to cell death   

 d) Phototoxic furanocoumarins are especially abundant in members of the Umbelliferae family, including celery, parsnip, and parsley.

 Which of the following combination from the above statements is correct?

TLS Online TPP Program

#Question id: 13065

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Agglutination of antigens by utilising specific antibodies can only occur, if

TLS Online TPP Program

#Question id: 13066

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Precision will be reduced, but yield will be increased Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following is a codominant marker?

TLS Online TPP Program

#Question id: 13067

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

The first immunoassay formats described were methods based on the ____ reaction, which is characterised either by gel formation in a liquid phase or as an opaque band in an agar plate assay.

TLS Online TPP Program

#Question id: 13068

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Coating antibody; which actively traps antigen

TLS Online TPP Program

#Question id: 13069

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

A negative agglutination test may NOT indicate the

TLS Online TPP Program

#Question id: 13070

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Antibodies raised against an antigen from one organism will react to a greater or lesser degree with similar antigens from a related organism, depending on how many surface epitopes they share. This has given rise to a systematic method of identification of microorganisms known as______?