Practice Questions

TLS Online TPP Program

#Question id: 3018

In the growth curve of a bacteria population, the bacteria are rapidly increasing in number in the

#Section 2: General Biology

lag phase.
exponential (log) phase.
stationary phase.
decline phase.

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TLS Online TPP Program

#Question id: 13089

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

What is the Colony 2’s plasmid is;

TLS Online TPP Program

#Question id: 14173

#Section 5: Bioprocess Engineering and Process Biotechnology

The growth of baker’s yeast (S. cerevisiae) on glucose may be simply described by the following equation:

In a batch reactor of volume 10^5 l, the final desired yeast concentration is 50 gdw/l. Using the above reaction stoichiometry. Determine the yield coefficient YX/O2 (biomass/oxygen)?

TLS Online TPP Program

#Question id: 10550

#Section 6: Plant, Animal and Microbial Biotechnology

Plants, in turn, evolved a second line of defense that recognize these intracellular effectors and trigger defense responses to render them harmless. As a result, plants possess a second type of immunity called,

TLS Online TPP Program

#Question id: 3591

#Section 3: Genetics, Cellular and Molecular Biology

Consider three yellow, round peas, labeled A, B, and C. Each was grown into a plant and crossed to a plant grown from a green, wrinkled pea. Exactly 100 peas issuing from each cross were sorted into phenotypic classes as follows

A: 51 yellow, round

49 green, round

B: 100 yellow, round

C: 24 yellow, round

26 yellow, wrinkled

25 green, round

25 green, wrinkled

What were the genotypes of A, B, and C respectively?

TLS Online TPP Program

#Question id: 15459

#Section 6: Plant, Animal and Microbial Biotechnology

In the growth curve of a bacteria population, the bacteria are rapidly increasing in number in the