An enzyme is produced for manufacturing a sun protection lotion. Given kinetic data for the enzyme reaction with v_max= 2.5mmol M^-3 S^-1 Km= 8.9 mM and So = 12 mM, what would be the time required for 95% conversion in a batch bioreactor? 

#Question id: 14190

#Section 5: Bioprocess Engineering and Process Biotechnology

Aerobic degradation of an organic compound by a mixed culture of organisms in waste water can be represented by the following reaction

Determine RQ for the organisms

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#Question id: 14191

#Section 5: Bioprocess Engineering and Process Biotechnology

Consider a 1000-l CSTR in which biomass is being produced with glucose as the substrate. The microbial system follows a Monod relationship withn µ_m = 0.4 h^-1 , K_S = 1.5 g/l (an unusu ally high value), and the yield factor Y_X/S = 0.5 g biomass/g substrate consumed. If normal operation is with a sterile feed containing 10 g/l glucose at a rate of 100 l/h. What is the specific biomass production rate (g/l-h) at steady state?

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#Question id: 14192

#Section 5: Bioprocess Engineering and Process Biotechnology

In a two-stage chemostat system, the volumes of the first and second reactors are V₁ = 500 l and V₂ = 300 l, respectively. The first reactor is used for biomass production and the second is for a secondary metabolite formation. The feed flow rate to the first reactor is F = 100 l/h, and the glucose concentration in the feed is S = 5.0 g/l. Use the following constants for the cells.

Determine cell concentration in the effluent of the first stage.

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#Question id: 14193

#Section 5: Bioprocess Engineering and Process Biotechnology

In a two-stage chemostat system, the volumes of the first and second reactors are V₁ = 500 l and V₂ = 300 l, respectively. The first reactor is used for biomass production and the second is for a secondary metabolite formation. The feed flow rate to the first reactor is F = 100 l/h, and the glucose concentration in the feed is S = 5.0 g/l. Use the following constants for the cells.

Determine glucose concentration in the effluent of the first stage. _____________________

#Question id: 14194

#Section 5: Bioprocess Engineering and Process Biotechnology

Penicillin is produced by P. chrysogenum in a fed-batch culture with the intermittent addition of glucose solution to the culture medium. The initial culture volume at quasi-steady state is V₀ = 500 l, and glucose-containing nutrient solution is added with a flow rate of F = 50 l/h. Glucose concentration in the feed solution and initial cell concentration are S₀ = 300 g/l and X₀ = 20 g/l, respectively. The kinetic and yield coefficients of the organism are mm = 0.2 h^-1 , K_S = 0.5 g/l, and Y_X/S = 0.3 g dw/g glucose. Determine the culture volume at t = 10 h

#Question id: 14195

#Section 5: Bioprocess Engineering and Process Biotechnology

Penicillin is produced by P. chrysogenum in a fed-batch culture with the intermittent addition of glucose solution to the culture medium. The initial culture volume at quasi-steady state is V₀ = 500 l, and glucose-containing nutrient solution is added with a flow rate of F = 50 l/h. Glucose concentration in the feed solution and initial cell concentration are S₀ = 300 g/l and X₀ = 20 g/l, respectively. The kinetic and yield coefficients of the organism are mm = 0.2 h^-1 , K_S = 0.5 g/l, and Y_X/S = 0.3 g dw/g glucose. Determine the concentration of glucose at t = 10 h at quasi-steady state