Find the chance of throwing at least one ace in a simple throw with two dice numbered 1 to 6.

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#Question id: 14337

#Section 5: Bioprocess Engineering and Process Biotechnology

A fermentation broth with viscosity 10 −2 Pa s and density 1000 kg m − 3 is agitated in a 2.7 m^ 3 baffled tank using a Rushton turbine with diameter 0.5 m and stirred speed 1 s ^−1. Estimate the mixing time.

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#Question id: 14485

#Section 1: Engineering Mathematics

In a race where 12 horses are running, the chance that horse A will win is 1/6, that B will win is 1/10 and that C will win is 1/8. Assuming that a dead heat is impossible the chance that one of them will win

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#Question id: 3001

#Section 2: General Biology

Which of the following participate in heat resistance of endospore?

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#Question id: 3760

#Section 3: Genetics, Cellular and Molecular Biology

In E. coli, what is the function of DNA polymerase III?

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#Question id: 13093

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

You are studying a specific gene in yeast, and you want to express that yeast gene in E. coli. Your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                                    

You transform your ligation planned in which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously, into bacteria and plate the bacteria on Petri plates containing ampicillin. (You actually transform six different ligation mixtures, which are described below, into six different populations of cells, and plate each transformation onto a different plate, because you want to do all of the correct controls.) The next day you come in to lab to look at how many colonies of bacteria are on each plate. You are really excited, because the number of colonies you see on each plate tells you that the entire procedure worked! Which of the three following patterns of number of colonies did you see in order to conclude that you had a successful transformation?

In this table, DV = digested vector. DYG = digested yeast genome.