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#Question id: 15143


Which one of the following is a depiction of the GenBank sequence entry format?

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology
  1. >YCZ2_YEAST protein in HMR 3' region MKAVVIEDGKAVVKEGVPIPELEEGFV
    GNPTDWAHIDYKVGPQSILGCDAAGQG*

  2. BASE COUNT 215 A 224 G 263 G 250 T ORIGIN Filename, Length of sequence, Date, ...
    1 GAATTCGATA AATCTCTGGT TTATTGTGCA 51 CTTTGCTGTA AGCATAACTG CAGGGGGCGG
  3. LOCUS name of locus, length and type of sequence. classification of organism, data of entry
    DEFINITION description of entry KEYWORDS key words for cross referencing this entry
    SOURCE ...
    ORGANISM...
    REFERENCE...
    COMMENT...
    FEATURES...
    BASE COUNT...
    ORIGIN text indicating start of sequence
    1 GAATTCGATA AATCTCTGGT TTATTGTGCA 51 CTTTGCTGTA AGCATAACTG CAGGGGGCGG //

  4.  >P1; ILEC
     lexA repressor Escherichia coli
    MKALTARQQEVFDLIRDHISQTGMPPTRAE IAQRLGFRSPNAAEEHLKALARKGVIEIVS

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TLS Online TPP Program

#Question id: 635

#Section 2: General Biology

The enymatic rate constant (kcat/Km) of orotidine 5ʹ-phosphate decarboxylase is 6 x 107 M-1s-1 and the nonenzymatic rate constant (kn) is 3 x 10-16 s-1.  What is the value of the enzymeʹs catalytic proficiency?

TLS Online TPP Program

#Question id: 636

#Section 2: General Biology

DNA polymerase contains a lysine residue that is important for binding to DNA. Mutations were found that converted this lysine to either glutamate, glycine, valine, or arginine. Which mutations would be predicted to be the most and least harmful to the ability of the enzyme to bind DNA?

Most

Least

(A)

Valine

Aspartate

(B)

Glycine

Arginine

(C)

Arginine

Glycine

(D)

Glutamate

Arginine

TLS Online TPP Program

#Question id: 638

#Section 2: General Biology

Match the following class of enzymes with them

EnzymeClass of enzyme
A. Hexokinase1
B. Phosphoglucoisomerase2
C. Glyceraldehyde 3-phosphate dehydrogenase3
D. Phosphoglycerate mutase4
E. Enolase5
F. Aldolase

TLS Online TPP Program

#Question id: 639

#Section 2: General Biology

Match the coenzymes in column I enzymes to class of enzymes in column II

EnzymeReaction Type
A. Phosphofructokinase1. Redox reaction
B. Enolase2. Phosphoryl shift
C. Phosphoglycerate mutase3. Dehydration
D. Glyceraldehyde 3 Phosphate dehydrogenase4. Phosphoryl transfer

TLS Online TPP Program

#Question id: 640

#Section 2: General Biology

The data in the table above were collected for an enzyme-catalyzed reaction. The Km for this enzyme is approximately

TLS Online TPP Program

#Question id: 641

#Section 2: General Biology

Which of the following does NOT describe a mechanism that cells use to regulate enzyme activities?

A. Cells control enzyme activity by phosphorylation and dephosphorylation.

B. Cells control enzyme activity by the binding of small molecules.

C. Cells control the rates of diffusion of substrates to enzymes.

D. Cells control the rates of enzyme degradation.

E. Cells control the rates of enzyme synthesis.

F. Cells control the targeting of enzymes to specific organelles.