TLS Online TPP Program

#Question id: 15536


Which of the following is incorrect regarding the progressive alignment method?

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology
  1. It speeds up the alignment of multiple sequences through a multistep process.
  2. It first conducts pair wise alignments for each possible pair of sequences using the Needleman–Wunsch global alignment method and records these similarity scores from the pair wise comparisons
  3. The scores can either be percent identity or similarity scores based on a particular substitution matrix.
  4. Its drawback is it slows down the alignment of multiple sequences through a single step process.
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TLS Online TPP Program

#Question id: 14189

#Section 5: Bioprocess Engineering and Process Biotechnology

Aerobic degradation of an organic compound by a mixed culture of organisms in waste water can be represented by the following reaction

               

Determine the degree of reductions for bacteria.

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TLS Online TPP Program

#Question id: 14190

#Section 5: Bioprocess Engineering and Process Biotechnology

Aerobic degradation of an organic compound by a mixed culture of organisms in waste water can be represented by the following reaction

               

Determine RQ for the organisms

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TLS Online TPP Program

#Question id: 14191

#Section 5: Bioprocess Engineering and Process Biotechnology

Consider a 1000-l CSTR in which biomass is being produced with glucose as the substrate. The microbial system follows a Monod relationship withn µm = 0.4 h-1 , KS = 1.5 g/l (an unusu ally high value), and the yield factor YX/S = 0.5 g biomass/g substrate consumed. If normal operation is with a sterile feed containing 10 g/l glucose at a rate of 100 l/h. What is the specific biomass production rate (g/l-h) at steady state?

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TLS Online TPP Program

#Question id: 14192

#Section 5: Bioprocess Engineering and Process Biotechnology

In a two-stage chemostat system, the volumes of the first and second reactors are V1 = 500 l and V2 = 300 l, respectively. The first reactor is used for biomass production and the second is for a secondary metabolite formation. The feed flow rate to the first reactor is F = 100 l/h, and the glucose concentration in the feed is S = 5.0 g/l. Use the following constants for the cells.



Determine cell concentration in the effluent of the first stage.

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TLS Online TPP Program

#Question id: 14193

#Section 5: Bioprocess Engineering and Process Biotechnology

In a two-stage chemostat system, the volumes of the first and second reactors are V1 = 500 l and V2 = 300 l, respectively. The first reactor is used for biomass production and the second is for a secondary metabolite formation. The feed flow rate to the first reactor is F = 100 l/h, and the glucose concentration in the feed is S = 5.0 g/l. Use the following constants for the cells.



Determine glucose concentration in the effluent of the first stage. _____________________

TLS Online TPP Program

#Question id: 14194

#Section 5: Bioprocess Engineering and Process Biotechnology

Penicillin is produced by P. chrysogenum in a fed-batch culture with the intermittent addition of glucose solution to the culture medium. The initial culture volume at quasi-steady state is V0 = 500 l, and glucose-containing nutrient solution is added with a flow rate of F = 50 l/h. Glucose concentration in the feed solution and initial cell concentration are S0 = 300 g/l and X0 = 20 g/l, respectively. The kinetic and yield coefficients of the organism are mm = 0.2 h-1 , KS = 0.5 g/l, and YX/S = 0.3 g dw/g glucose. Determine the culture volume at t = 10 h