TLS Online TPP Program
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TLS Online TPP Program
#Question id: 14105
#Section 7: Recombinant DNA technology and Other Tools in Biotechnology
The first secondary database developed was
TLS Online TPP Program
#Question id: 14116
#Section 7: Recombinant DNA technology and Other Tools in Biotechnology
Which of the following statements are CORRECT when a protein sequence database is searched using the BLAST algorithm?
P. A larger E-value indicates higher sequence similarity
Q. E-value < 10^-10 indicates sequence homology
R. A higher BLAST score indicates higher sequence similarity
S. E-value > 10^10 indicates sequence homology
TLS Online TPP Program
#Question id: 3039
#Section 2: General Biology
An inoculated thioglycolate medium culture tube shows dense growth at the surface and turbidity throughout the rest of the tube. What is your conclusion?
TLS Online TPP Program
#Question id: 13054
#Section 7: Recombinant DNA technology and Other Tools in Biotechnology
Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
What would the expected effect be on a PCR reaction if the primers used were slightly shorter and more variable than the intended oligonucleotide sequences?
TLS Online TPP Program
#Question id: 4803
#Section 3: Genetics, Cellular and Molecular Biology
Linked genes: