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TLS Online TPP Program

#Question id: 15885

In batch fermentation___

#Section 6: Plant, Animal and Microbial Biotechnology
  1. Substrates are added to the system all at once and runs until product is harvested
  2. Nutrient are continuously fed into the reactor and the product is siphoned off during the run
  3. New batches of microorganisms are screened for increase yield
  4. Small-scale production is used to synthesize product
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TLS Online TPP Program

#Question id: 16776

#Section 1: Engineering Mathematics

If the Mean and Mode are 25, then find the Median.
TLS Online TPP Program

#Question id: 13067

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

The first immunoassay formats described were methods based on the ____ reaction, which is characterised either by gel formation in a liquid phase or as an opaque band in an agar plate assay.
TLS Online TPP Program

#Question id: 3794

#Section 3: Genetics, Cellular and Molecular Biology

An input of energy is not required for which of the following steps of replication?
TLS Online TPP Program

#Question id: 14220

#Section 5: Bioprocess Engineering and Process Biotechnology

A value of kLa = 30 h^-1 has been determined for a fermenter at its maximum practical agitator rotational speed and with air being sparged at 0.5 l gas/l reactor volume-min. E. coli with a qO2 of 10 mmol O2/g-dry wt-h are to be cultured. The critical dissolved oxygen concentration is 0.2 mg/l. The solubility of oxygen from air in the fermentation broth is 7.3 mg/l at 30⁰C.
What maximum concentration of E. coli can be sustained in this fermenter under aerobic conditions?
TLS Online TPP Program

#Question id: 14240

#Section 5: Bioprocess Engineering and Process Biotechnology

A 200-1itre stirred fermenter contains a batch culture of Bacillus subtilis bacteria at 28⁰C Air at 20⁰C is pumped into the vessel at a rate of 1 vvm; (vvm stands for volume of gas per volume of liquid per minute). The average pressure in the fermenter is 1 atm. The volumetric flow rate of off-gas from the fermenter is measured as 189 l min-1. The exit gas stream is analysed for oxygen and is found to contain 20.1% 02. The dissolved-oxygen concentration in the broth is measured using an oxygen electrode as 52% air saturation. The solubility of oxygen in the fermentation broth at 28⁰C and 1 atm air pressure is 7.8 x 10-3 kg m-3. Determine the value of kLa for the system ____________