TLS Online TPP Program
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TLS Online TPP Program
#Question id: 12910
#Section 7: Recombinant DNA technology and Other Tools in Biotechnology
Match the following:
| A. RNA analysis | i. Sexing or cell mutation sites | a. Expressed Sequence tag |
| B. Forensic science | ii. mRNA analysis | b. Analysis of DNA from blood |
| C. Gene discovery | iii. DNA profiling | c. Active latent viral infections |
| D. Single-cell analysis | iv. RT–PCR | d. Sex determination of unborn |
TLS Online TPP Program
#Question id: 12911
#Section 7: Recombinant DNA technology and Other Tools in Biotechnology
Match the following:
| A. DNA polymerases | i. Alkaline phosphatase | a. RNA-dependent DNA polymerase, used in cDNA synthesis |
| B. Reverse transcriptase | ii. T4 DNA ligase | b. Thermostable DNA polymerase used in PCR |
| C. Ligases | iii. AMV-RT | c. Removes 5'-phosphates from DNA and RNA |
| D. Phosphatases | iv. Taq DNA pol | d. Links 5'-phosphate and 3'-hydroxyl ends via phosphodiester bond |
TLS Online TPP Program
#Question id: 12912
#Section 7: Recombinant DNA technology and Other Tools in Biotechnology
Name the enzyme which separates the double-helical DNA into single strands.
TLS Online TPP Program
#Question id: 12913
#Section 7: Recombinant DNA technology and Other Tools in Biotechnology
________ involves the size analysis of restriction fragments produced by several restriction enzymes individually and in combination.
TLS Online TPP Program
#Question id: 12914
#Section 7: Recombinant DNA technology and Other Tools in Biotechnology
In Electrophoresis of DNA the gel is first soaked in the alkali then it is transferred to the membrane so that the ________
TLS Online TPP Program
#Question id: 12915
#Section 7: Recombinant DNA technology and Other Tools in Biotechnology
Which of the following statement is not correct about PCR.