TLS Online TPP Program
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TLS Online TPP Program
#Question id: 15488
#Section 6: Plant, Animal and Microbial Biotechnology
IMP biosynthetic genes constitute the pur operon and are involved in Purines synthesis. Bacterial strain used for this
TLS Online TPP Program
#Question id: 28114
#Section 5: Bioprocess Engineering and Process Biotechnology
Which is the most frequently used impeller in the fermentation industry regarded as 6- flat-blade disc-mounted turbine?
TLS Online TPP Program
#Question id: 5255
#Section 3: Genetics, Cellular and Molecular Biology
Which of the following is a structure that permits conjugation to occur?
TLS Online TPP Program
#Question id: 3612
#Section 3: Genetics, Cellular and Molecular Biology
Multiple alleles control inheritance of
TLS Online TPP Program
#Question id: 13090
#Section 7: Recombinant DNA technology and Other Tools in Biotechnology
To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.
The distance between each tick mark placed on the line above is 100 bases in length
Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’
The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.
You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.
What is the Colony 3’s plasmid is;