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TLS Online TPP Program

#Question id: 18683

Eukaryotic mRNA can be separated by using 

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology
  1. paper chromatography, 
  2. gas chromatography
  3. affinity chromatography
  4. ion exchange chromatography
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TLS Online TPP Program

#Question id: 13090

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 
The distance between each tick mark placed on the line above is 100 bases in length
Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 
The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.
                              
You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.
 
What is the Colony 3’s plasmid is;
TLS Online TPP Program

#Question id: 3758

#Section 3: Genetics, Cellular and Molecular Biology

 At a specific area of a chromosome, the sequence of nucleotides below is present where the chain opens to form a replication fork:

3' C C T A G G C T G C A A T C C 5'

An RNA primer is formed starting at the underlined T (T) of the template. Which of the following represents the primer sequence?
TLS Online TPP Program

#Question id: 14622

#Section 2: General Biology

Endospores are
TLS Online TPP Program

#Question id: 2123

#Section 2: General Biology

Which of these statements about the composition of membranes is true?
TLS Online TPP Program

#Question id: 14201

#Section 5: Bioprocess Engineering and Process Biotechnology

A fluidized-bed, immobilized-cell bioreactor is used for the conversion of glucose to ethanol by Z. mobilis cells immobilized in k-carrageenan gel beads. The dimensions of the bed are 10 cm (diameter) by 200 cm. Since the reactor is fed from the bottom of the column and because of CO2 gas evolution, substrate and cell concentrations decrease with the height of the column. The average cell concentration at the bottom of the column is X0 = 45 g/l, and the average cell concentration decreases with the column height according to the following equation:

                             

where Z is the column height (cm). The specific rate of substrate consumption is qS = 2 g S/g cells . h. The feed flow rate and glucose concentration in the feed are 5 l/h and 160 g glucose/l, respectively.

Determine the ethanol productivity (g/l . h) if YP/S = 0.48 g ethanol/g glucose.

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