The half-life (t1/2) of a first-order reaction is 0.950 s. What is the rate constant?

#Question id: 14295

#Section 5: Bioprocess Engineering and Process Biotechnology

Pseudomonas methylotrophus is used to produce single-cell protein from methanol in a 1000 m³ pressure-cycle airlift fermenter. The biomass yield from substrate is 0.41 g g^{- 1}, K_S is 0.7 mg l^{- 1}, and the maximum specific growth rate is 0.44 h^{- 1}. The medium contains 4% (w/v) methanol. A substrate conversion of 98% is desirable. The reactor may be operated either in batch or continuous mode. If operated in batch, an inoculum of 0.01% (w/v) is used and the downtime between batches is 20 h. If operated continuously, a downtime of 25 d is expected per year. Neglecting maintenance requirements, compare the annual biomass production from batch and continuous reactors?

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#Question id: 14192

#Section 5: Bioprocess Engineering and Process Biotechnology

In a two-stage chemostat system, the volumes of the first and second reactors are V₁ = 500 l and V₂ = 300 l, respectively. The first reactor is used for biomass production and the second is for a secondary metabolite formation. The feed flow rate to the first reactor is F = 100 l/h, and the glucose concentration in the feed is S = 5.0 g/l. Use the following constants for the cells.

Determine cell concentration in the effluent of the first stage.

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#Question id: 14354

#Section 5: Bioprocess Engineering and Process Biotechnology

deceleration phase comes after with phase in growth curve. 

#Question id: 8914

#Section 6: Plant, Animal and Microbial Biotechnology

#Question id: 23127

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology