In RNA, nucleotides are joined by which type of bond?

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#Question id: 1592

#Section 2: General Biology

Which of these molecules is incorrectly paired with a source?

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#Question id: 27325

#Section 5: Bioprocess Engineering and Process Biotechnology

if the initial substrate concentration is 15 g/L and initial biomass concentration is 0.1 g/L. The substrate is then consumed completely and produces 6.4 g/L of biomass. The yield coefficient will be

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#Question id: 5242

#Section 3: Genetics, Cellular and Molecular Biology

Auxotrohic bacteria plating on different medium, it is observed that colonies grow on minimal plus threonine only, no colony on minimal plus leucine and no colonies grow on minimal medium cystein only. What are the actual genotypes of auxotroph?

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#Question id: 15171

#Section 6: Plant, Animal and Microbial Biotechnology

Fermentative microorganisms form organic acids from carbohydrates, whereas chemolithotrophs like Thiobacillus oxidize reduced

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#Question id: 13093

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

You are studying a specific gene in yeast, and you want to express that yeast gene in E. coli. Your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                                    

You transform your ligation planned in which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously, into bacteria and plate the bacteria on Petri plates containing ampicillin. (You actually transform six different ligation mixtures, which are described below, into six different populations of cells, and plate each transformation onto a different plate, because you want to do all of the correct controls.) The next day you come in to lab to look at how many colonies of bacteria are on each plate. You are really excited, because the number of colonies you see on each plate tells you that the entire procedure worked! Which of the three following patterns of number of colonies did you see in order to conclude that you had a successful transformation?

In this table, DV = digested vector. DYG = digested yeast genome.