What is the PAM site sequence for Cas9?

TLS Online TPP Program

#Question id: 12911

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Match the following:

A. DNA polymerases              i. Alkaline phosphatase        a.RNA-dependent DNA polymerase, used in cDNA synthesis

B. Reverse transcriptase        ii. T4 DNA ligase                    b. Thermostable DNA polymerase used in PCR

C. Ligases                                 iii.  AMV-RT                            c. Removes 5′-phosphates from DNA and RNA

D. Phosphatases                     iv. Taq DNA pol                      d. Links 5′-phosphate and 3′-hydroxyl ends via phosphodiester bond

TLS Online TPP Program

#Question id: 12912

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Name the enzyme which separates the double-helical DNA into single strands.

TLS Online TPP Program

#Question id: 12913

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

________  involves the size analysis of restriction fragments produced by several restriction enzymes individually and in combination. 

TLS Online TPP Program

#Question id: 12914

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

 In Electrophoresis of DNA the gel is first soaked in the alkali then it is transferred to the membrane so that the ________

TLS Online TPP Program

#Question id: 12915

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Which of the following statement is not correct about PCR.

TLS Online TPP Program

#Question id: 12916

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Which statement is correct for Zoo blotting?