A double reciprocal plot was created from the specific growth rate and limiting-substrate concentration data obtained from a chemostat experiment. A linear regression gave values of 1.25 h and 100 mg hL-1 for the intercept and slope, respectively.

(a) The respective values of the Monod kinetic constants μm (h-1) and K. (mg L-¹) are as follows:

#Question id: 3012

#Section 2: General Biology

Agar is a sulfated polymer

#Question id: 19929

#Section 5: Bioprocess Engineering and Process Biotechnology

#Question id: 14295

#Section 5: Bioprocess Engineering and Process Biotechnology

Pseudomonas methylotrophus is used to produce single-cell protein from methanol in a 1000 m³ pressure-cycle airlift fermenter. The biomass yield from substrate is 0.41 g g^{- 1}, K_S is 0.7 mg l^{- 1}, and the maximum specific growth rate is 0.44 h^{- 1}. The medium contains 4% (w/v) methanol. A substrate conversion of 98% is desirable. The reactor may be operated either in batch or continuous mode. If operated in batch, an inoculum of 0.01% (w/v) is used and the downtime between batches is 20 h. If operated continuously, a downtime of 25 d is expected per year. Neglecting maintenance requirements, compare the annual biomass production from batch and continuous reactors?

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#Question id: 2074

#Section 3: Genetics, Cellular and Molecular Biology

In some cells, there are many ion electrochemical gradients across the plasma membrane even though there are usually only one or two proton pumps present in the membrane. The gradients of the other ions are most likely accounted for by:

#Question id: 15682

#Section 6: Plant, Animal and Microbial Biotechnology