If ΔH= 158 kJ and ΔS=411 J/K. At what temperature will this reaction be spontaneous?

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#Question id: 3021

#Section 6: Plant, Animal and Microbial Biotechnology

The lag phase of the bacterial growth curve is marked by

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#Question id: 3861

#Section 3: Genetics, Cellular and Molecular Biology

_ are sequences that are removed from primary transcripts, and _ are sequences that remain present in mature mRNA molecules.

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#Question id: 2925

#Section 3: Genetics, Cellular and Molecular Biology

Mitotic cyclin levels in early Xenopus embryos are regulated by:

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#Question id: 14698

#Section 1: Engineering Mathematics

A bag has 13 red, 14 green and 15 black balls. The probability of getting exactly 2 blacks on pulling out 4 balls is P1. Now the number of each colour ball is doubled and 8 balls are pulled out.The probability of getting exactly 4 blacks is P2. Then

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#Question id: 13092

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously?