The Forces of Evolution: 

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#XL - T Zoology

van’t Hoff plot is used measure rate of unfolding of proteins

TLS Online TPP Program

#Id: 8773

#XL - T Zoology

Techniques for Measuring Stability

Absorbance (e.g. Trp, Tyr) 

Fluorescence (Trp)-difference in emission max & intensity.

CD (far or near UV) - (2o or 3o) 

NMR 

DSC (calorimetry) 

Urea gradient gels - difference in the migrating rates between F and U.

Catalytic activity 

Chromophoric or fluorophoric probes/FRET 

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#XL - T Zoology

The Cp heat capacity of a defined system is the amount of heat (usually expressed in calories, kilocalories, or joules) needed to raise the system's temperature by one degree (usually expressed in Celsius or Kelvin).

TLS Online TPP Program

#Id: 8775

#XL - T Zoology

Thermal stability of protein can be calculated

TLS Online TPP Program

#Id: 8776

#XL - T Zoology

TLS Online TPP Program

#Id: 8777

#XL - T Zoology

A.

1 - native

3 - MG

2, 4 - unfolded

B.

1 - native

3, 4 - MG

2 - unfolded