The Forces of Evolution: 

TLS Online TPP Program

#Id: 9658

#XL - T Zoology

Hormonal stimulation of GPCR leads to the activation of PLC (IP3/DAG pathway).

TLS Online TPP Program

#Id: 9659

#XL - T Zoology

GPCRs specifically activate the β isoform of PLC (PLCβ)

TLS Online TPP Program

#Id: 9660

#XL - T Zoology

Many RTKs and cytokine receptors specifically activate the γ isoform (PLCγ), which contains an SH2 domain.

TLS Online TPP Program

#Id: 9661

#XL - T Zoology

Activated RTKs and cytokine receptors promote PLCγ activity in two ways: 

By localizing PLCγ to the membrane

By phosphorylating PLCγ

TLS Online TPP Program

#Id: 9662

#XL - T Zoology

The SH2 domains of PLCγ bind to specific phosphotyrosines on the activated RTKs and cytokine receptors, thus positioning PLCγ close to PIP2.

TLS Online TPP Program

#Id: 9663

#XL - T Zoology

Kinase activity associated with receptor activation phosphorylates tyrosines on the bound PLCγ, enhancing its hydrolase activity.