A covalent bond is a chemical bond that occurs when two or more atoms share electrons. The sharing of electrons helps complete the outer shell, or valence shell, of both atoms. The sharing of electrons creates a stable balance of attractive and repulsive forces between atoms.

TLS Online TPP Program

#Id: 8562

#XL - Q Biochemistry

In neither case does the inhibitor compete with the substrate for the same binding site, so the inhibition cannot be overcome by increasing the substrate concentration.

TLS Online TPP Program

#Id: 8563

#XL - Q Biochemistry

Substrate-binding could cause a conformational change to take place in the enzyme and reveal an inhibitor binding site, or the inhibitor could bind directly to the enzyme-bound substrate. 

TLS Online TPP Program

#Id: 8564

#XL - Q Biochemistry

TLS Online TPP Program

#Id: 8565

#XL - Q Biochemistry

In the simplest possible model, simple linear non-competitive inhibition, the substrate does not affect inhibitor-binding. 

TLS Online TPP Program

#Id: 8566

#XL - Q Biochemistry

Under these conditions, the reactions have an identical dissociation constant Ki , again called the inhibitor constant. 

TLS Online TPP Program

#Id: 8568

#XL - Q Biochemistry

In the presence of a non-competitive inhibitor which will bind equally well to E or to ES- Vmax decreases but not Km