Membrane contact sites (MCSs)

Mitochondria establish MCSs with many other organelles, including ER, lysosomes, peroxisomes, the plasma membrane, the Golgi, endosomes, and even lipid droplets.

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#XL - Q Biochemistry

Membrane contact sites (MCSs)

Mitochondria establish MCSs with many other organelles, including ER, lysosomes, peroxisomes, the plasma membrane, the Golgi, endosomes, and even lipid droplets.

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#XL - T Zoology

Cohesins are composed of four subunits: 

Smc1, Smc3, Scc1, and Scc3

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Smc1 and Smc3 are members of the structural

maintenance of chromosomes (SMC) protein family, characterized by globular domains at the N- & C-termini connected by a long α helix, bind and hydrolyze ATP.

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The ATPase domains interact with the proteins

Scc1 and Scc3, which together form a cohesin ring structure which embrace one or both copies of the replicated DNA.

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Cohesin-mediated cohesion between sister chromatids is established by a two-step process:

1.Loading and unloading of cohesins

2. Stabilization of cohesins

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During G1, 

cohesins associate with chromosomes by cohesin loading factors Scc2 and Scc4 and unloaded continuously by a cohesin associated complex Pds5 and Wap1 proteins. 

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During S phase

DNA-bound G1 cohesins Convert into cohesive complexes by cohesin acetyl transferases (CoATs)

which acetylate Smc3 subunit & prevents unloading of cohesins by Pds5-Wapl, thereby stabilizing the cohesins on chromosomes.