Negatively charged amino acids have four ionisations zones

1. 1--------pK1 – charge +1

2. pK1---------pKr charge 0

3. pKr---------pK2 charge -1

4. pK2---------14 charge -2

TLS Online TPP Program

#Id: 8153

#XL - Q Biochemistry

A second function of small GTPases in vesicle formation is in the pinching off of a completed vesicle from the parent membrane

TLS Online TPP Program

#Id: 8152

#XL - Q Biochemistry

After the vesicle coat is complete, the Sec23 coat subunit promotes GTP hydrolysis causes disassembly of the coat.

TLS Online TPP Program

#Id: 8151

#XL - Q Biochemistry

Sec12

acts as a GEF factor for Sar1

TLS Online TPP Program

#Id: 8150

#XL - Q Biochemistry

Sar1 is the only GTPase switch protein activated at the ER membrane

TLS Online TPP Program

#Id: 8149

#XL - Q Biochemistry

ARF and Sar1 similar to Ras

TLS Online TPP Program

#Id: 8148

#XL - Q Biochemistry

Depolymerization of the clathrin coat into triskelions  by Cytosolic Hsp70