Two major classes of actin-nucleating proteins:

1. Formins nucleate the assembly of unbranched filaments 

2. Arp2/3 complex nucleates the assembly of branched actin networks

TLS Online TPP Program

#Id: 8942

#XL - T Zoology

In S. cerevisiae, during interphase and early mitosis, Cdc14 is sequestered and inactivated in the nucleolus. 

TLS Online TPP Program

#Id: 8943

#XL - T Zoology

Inactive Tem1-GDP associates with the spindle pole body nearest to the bud as soon as the mitotic spindle forms

TLS Online TPP Program

#Id: 8944

#XL - T Zoology

If chromosome segregation occurs properly, extension of the spindle microtubules inserts the daughter SPB into the bud, causing Tem1 to be activated by an unknown mechanism.

TLS Online TPP Program

#Id: 8945

#XL - T Zoology

Tem1-GTP activates a protein kinase cascade, which promotes the release of active Cdc14 from the nucleolus and exit from mitosis. 

TLS Online TPP Program

#Id: 8946

#XL - T Zoology

An inhibitor of the GTPase, called Kin4, localizes to the parent cell but is absent from the bud.

TLS Online TPP Program

#Id: 8947

#XL - T Zoology

An inhibitor of Kin4, called Lte1, localizes to the bud but is absent from the parent cell; it inhibits any residual Kin4 that leaks into the bud.