Ste7, phosphorylates and activates Fus3,  a serine/threonine kinase

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Pyruvate Carboxylase

a mitochondrial enzyme that requires the coenzyme biotin, converts the pyruvate to oxaloacetate

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The oxaloacetate is converted to PEP by phosphoenolpyruvate carboxykinase. This Mg2+ dependent reaction requires GTP as the phosphoryl group donor.

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Transketolase requires the cofactor thiamine pyrophosphate (TPP)

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Acetyl-CoA and NADH are potent allosteric inhibitors of the enzyme. 

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Acetyl-CoA inhibits the transacetylase component whereas NADH inhibits the dihydrolipoyl dehydrogenase component. 

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The reaction catalyzed by citrate synthase is essentially a Claisen condensation, involving a thioester (acetyl-CoA) and a ketone (oxaloacetate)