Following statements are regarding to receptor serine kinases.

A. The two serines in each Smad3 that were phosphorylated by the RI receptor kinase bind to phosphoserine-binding sites in the MH1 domains of a Smad3 or a Smad4, forming a stable complex containing two molecules of phosphorylated Smad3 (or Smad2) and one molecule of the co-Smad (Smad4).

B. The bound importin then mediates translocation of the heteromeric R-Smad/co-Smad complex into the nucleus.

C. After importin dissociates inside the nucleus, the Smad3/Smad4 (or Smad2/Smad4) complex binds to other transcription factors to activate transcription of specific target genes.

D. Transcription of the PAI-1 gene requires formation of a complex of the transcription factor TFE3 with the R-Smad/co-Smad (Smad3/Smad4) complex and binding of all these proteins to specific sequences within the regulatory region of the PAI-1 gene.

E. Within the nucleus, R-Smads are further modified by phosphorylation of their linker domains, monoubiquitinylation of their MH1 domains, acetylation of their MH2 domains, and dephosphorylation of the C-terminal serines by nuclear phosphatases.

Which of the following combination is incorrect?

#Question id: 10489

#Unit 6. System Physiology – Plant

Malate anions, by contrast, are synthesized in the guard cell cytosol, in a metabolic pathway that uses carbon skeletons generated by

#Question id: 10488

#Unit 6. System Physiology – Plant

Blue light activates a proton pump at the guard cell plasma membrane. The relationship between proton pumping at the guard cell plasma membrane and stomatal opening is evident from the observations that;

 a.) Orthovandate inhibits the fusicoccin-stimulated opening

 b.) fusicoccin stimulates both proton extrusion from guard cell protoplasts and stomatal opening

 c.) CCCP inhibits the fusicoccin-stimulated opening

 d.) CCCP, a proton ionophore that makes the plasma membrane highly permeable to protons resulting stomatal opening

 Which one of the following observation from the above statements should  be correct?

#Question id: 10487

#Unit 6. System Physiology – Plant

During the patch clamp experiments measure outward electric current in picoamps, pA at the plasma membrane of a guard cell protoplast stimulated by two points A and B showing in the graph.

What is A and B

#Question id: 10486

#Unit 6. System Physiology – Plant

A patch clamp study shows a guard cell protoplast treated in the dark with the fungal toxin fusicoccin which is a well-characterized,

#Question id: 10485

#Unit 6. System Physiology – Plant

What is the role of blue light in terms of stomatal study?

a.) Blue light activates a proton-pumping ATPase in the guard cell plasma membrane and leading to stomatal opening

b.) blue-light stimulation lowers the pH of the apoplastic space surrounding the guard cells, and generates the driving force needed for ion uptake and stomatal opening

c.) The sensitivity of guard cells to blue light increases as a function of their zeaxanthin concentration

d.) npq1 mutant opens at higher fluence rates of blue light, whereas the phot1/phot2 mutant fails to show any blue light–stimulated opening

Which one of the following combination is correct?

#Question id: 10483

#Unit 6. System Physiology – Plant

Given below some facts regarding the stomal responses in light condition. Which one of the following would be INCORRECT?