A generalized transducing phage is used to transduce an a- b- c- d- e- recipient strain of E. coli with an a+ b+c+d+ e+ donor. The recipient culture is plated on various media with the results shown in the table below What can you conclude about the linkage and order of the genes?

#Question id: 801

#Unit 1. Molecules and their Interaction Relevant to Biology

Identify the type of DNA from the structures given below and match the correct form with its characteristics described.

#Question id: 800

#Unit 1. Molecules and their Interaction Relevant to Biology

Following table lists the two major forms of DNA duplexes, conformation of base attached to the sugar and the nature of major and minor grooves.

#Question id: 799

#Unit 1. Molecules and their Interaction Relevant to Biology

The two cleavage reactions required to generate the miRNA from these primary transcripts are mediated by two distinct RNases. A characteristic of both these enzymes is that they recognize and cleave RNAs on the basis of the structure of their substrates rather than their specific sequence. Which of the following statement is INCORRECT regarding the cleavage reactions that produces a mature RNA?

#Question id: 798

#Unit 1. Molecules and their Interaction Relevant to Biology

Match the structural properties of DNA with the respective types of DNA in which they are found.

               COLUMN – I                                             COLUMN – II

A.        Pitch per turn of helix                                     1. 45.6 angstrom                   

                                                                                      2. 24.6 angstrom                               

 3. 33.2 angstrom

B.        Helix packing diameter                                 1. 23.7 angstrom            

                                                                                     2. 18.4 angstrom            

3. 25.5 angstrom

C.        Major-groove proportions                            1. Flattened out on helix surface

                                                                                      2. Extremely narrow but very deep

 3. Wide and of intermediate depth

D.        Minor-groove proportions                            1. Very broad but shallow

                                                                                     2. Extremely narrow but very deep

                                                                                    3. Narrow and of intermediate depth

#Question id: 797

#Unit 1. Molecules and their Interaction Relevant to Biology

With respect to microRNAs, which, if any, of the following statements, is FALSE?)

A. MicroRNA genes often occur in gene clusters within introns of protein-coding genes and are usually transcribed by RNA polymerase II.

B. Like an mRNA a miRNA is initially produced as a larger precursor RNA that, like the great majority of mRNAs, usually has a 5’ cap and a poly(A) tail.

C. MicroRNAs are produced by germ-line cells only.

D. A miRNA is initially produced as a duplex RNA but in order to work it needs to be converted into a single-strand RNA.

E. MicroRNAs usually work as transcription factors.

#Question id: 796

#Unit 1. Molecules and their Interaction Relevant to Biology

Which statement best describes the distinction between the purposes of the two RNAi pathways within the eukaryotic cell?