Companion cells transport of photosynthetic products from producing cells in mature leaves to the sieve elements in the minor veins of the leaf. There are at least three different types of companion cells in the minor veins of mature, exporting leaves, All three cell types have dense cytoplasm and abundant mitochondria;

TLS Online TPP Program

#Question id: 14360

#SCPH05 I Biotechnology

1 pg of an enzyme (Mr 160 000) converts 0.192 pmol/min. This enzyme is made up of four subunits. Calculate turnover number 

TLS Online TPP Program

#Question id: 14361

#SCPH05 I Biotechnology

1 m of an enzyme (Mr 160 000) converts 0.192 i.imol/min. This enzyme is made up of four  subunits. Calculate catalytic centre activity

TLS Online TPP Program

#Question id: 14362

#SCPH05 I Biotechnology

An experiment requires the use of 5 x 107 units of enzyme. The price for the enzyme is quoted for katal amounts. Allowing for a 20% excess for wastage, pipetting, and so forth, what approximate round number of katals of enzyme would you purchase? 

TLS Online TPP Program

#Question id: 14363

#SCPH05 I Biotechnology

Enzyme activity was monitored over the last two stages of an enzyme extraction-separation process. 0.4 g protein, from Stage 5, converted 46 mmol substrate in 10 sec, and 0.02 g protein, from Stage 6, converted 49 mmol in 30 sec. Calculate the specific activities in units/g at each of the two steps 

TLS Online TPP Program

#Question id: 14364

#SCPH05 I Biotechnology

Enzyme activity was monitored over the last two stages of an enzyme extraction-separation process. 0.4 g protein, from Stage 5, converted 46 mmol substrate in 10 sec, and 0.02 g protein, from Stage 6, converted 49 mmol in 30 sec. Calculate % increase in activity obtained over the two step  

TLS Online TPP Program

#Question id: 14365

#SCPH05 I Biotechnology

0.1 mg of a quaternary structured enzyme (Mr 486 000) with four active sites converts 48 iimol substrate/10 sec. Calculate turnover number (min-1)