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TLS Online TPP Program
#Question id: 13062
#SCPH01 Biochemistry
Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
A restriction enzyme cleaves a circular plasmid vector to produce fragments : 1kb, 0.3kb, 2kb, 1kb and 0.3kb. How many bands will be visible on agarose gel?
TLS Online TPP Program
#Question id: 3904
#SCPH28 | Zoology
Which statement about RNA polymerase is false?
TLS Online TPP Program
#Question id: 2091
#I Life Science/ Life Sciences Group – I-V
Proteins can be attached to the phospholipid bilayer plasma membrane by covalently linked hydrocarbon groups. Which of the following mechanisms are not employed in this anchoring?
TLS Online TPP Program
#Question id: 14827
#SCPH05 I Biotechnology
The structures used for motility in both eukaryotes and prokaryotes are
TLS Online TPP Program
#Question id: 2659
#SCPH05 I Biotechnology
The correct option regarding the lac operon in E.coli from the following is