Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Agglutination of antigens by utilising specific antibodies can only occur, if

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#Question id: 2621

#SCPH05 I Biotechnology

The leaky expression of lac genes ensures that,

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#Question id: 2652

#SCPH05 I Biotechnology

 Which of these acts as an inducer of the lac operon?

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#Question id: 20986

#SCPH01 Biochemistry

In consistent fashion the btex hydrocarbon decay is linked with __________as the terminal electron acceptor.

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#Question id: 854

#SCPH06 I Botany

Many conditions including type 2 diabetes, Alzheimer  disease, Huntington disease and parkinson disease are associated with the misfolding mechanism a soluble protein that is normally secreted from the cell is secreted in the missfold state and converted into an insoluble extracellular fibre the diseases are correctly referred to as

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#Question id: 18223

#SCPH28 | Zoology

The origin of indirect evidence of life, no recognizable fossils except bacteria (microfossils) was during which geological eras?