Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Efficiency during ligation of blunt ended insert and vector cannot be cannot be enhanced by means of…..

#Question id: 7767

#I Life Science/ Life Sciences Group – I-V

How can evolution select for phenotypes that postpone reproduction or sexual maturity?” There is often a trade-off between reproduction and maintenance, and in many species reproduction and senescence are closely linked. Recent studies of mice, Caenorhabditis elegans, and Drosophila suggest that there is a conserved genetic pathway that regulates aging and that it can indeed be selected for. This pathway involves the response to insulin and insulin-like growth factors, Name of these transcription factors are

#Question id: 6996

#SCPH01 Biochemistry

During metamorphosis, a tadpole's tail is reduced in size by the process of ________.

#Question id: 2106

#I Life Science/ Life Sciences Group – I-V

When using immunofluorescence microscopy with labeled Annexin V antibodies you found that there is a change in the distribution of the fluorescence when the cells were treated with tumor necrosis factor, an apoptosis-inducing agent. Your data show that this increase in Annexin V fluorescence increases specifically in the:

#Question id: 11010

#I Life Science/ Life Sciences Group – I-V

Which of the following best explains how sympathetic stimulation affects the heart?

#Question id: 15911

#I Life Science/ Life Sciences Group – I-V