#Question id: 13076
#SCPH28 | Zoology
Restriction fragment length polymorphism denotes that a
single restriction enzyme produces fragments of different lengths from the same
stretch of genomic DNA of different strains of a species or from different
related species. RFLPs are detected as follows:
i. Large
molecular weight genomic DNA is isolated from several strains or related
species;
ii. The
fragments in these digests are separated through electrophoresis
iii. These
'DNAs are then digested with a selected restriction enzyme
iv. Exposed
to a suitably radio-labelled appropriate DNA probe under conditions favouring
DNA: DNA hybridization
v. The
resulting gel lanes are transferred and fixed to a suitable solid support and
vi. The free
probes are removed
vii. The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.
#Question id: 3920
#SCPH28 | Zoology
The genes that encode proteins are transcribed by RNA polymerase __.
#Question id: 8894
#SCPH06 I Botany
#Question id: 14311
#SCPH05 I Biotechnology
Consider following fig, which applies to a fed-batch system.
Assume at t = 0, V = 100 l, X = 2 g/l, m = 1 h-1 , S0 = 4 g/l, and S = 0.01 g/l. V is increased at a constant rate such that dV/dt = 20 l/h = F (or flow rate) and X is constant at all times. What is ยต at t = 5 h?
#Question id: 4150
#SCPH05 I Biotechnology
Posttranslational glycosylation of proteins is inhibited specifically by: