Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following true about real time PCR?

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#Question id: 12903

#SCPH01 Biochemistry

A gene isolation system that uses adapted vectors termed as_______

TLS Online TPP Program

#Question id: 12903

#SCPH06 I Botany

A gene isolation system that uses adapted vectors termed as_______

TLS Online TPP Program

#Question id: 12903

#SCPH28 | Zoology

A gene isolation system that uses adapted vectors termed as_______

TLS Online TPP Program

#Question id: 12904

#SCPH01 Biochemistry

Match the following:

1. Southern Blotting                               i. Melting temperature of DNA strand

2. DNA microchips                                   ii.oligonucleotide matching to DNA sample

3. ASO-PCR                                                 iii.DNA hybridization to oligo arrays

4. DGGE                                                        iv. labeled probe hybridization to DNA  

TLS Online TPP Program

#Question id: 12904

#SCPH06 I Botany

Match the following:

1. Southern Blotting                               i. Melting temperature of DNA strand

2. DNA microchips                                   ii.oligonucleotide matching to DNA sample

3. ASO-PCR                                                 iii.DNA hybridization to oligo arrays

4. DGGE                                                        iv. labeled probe hybridization to DNA  

TLS Online TPP Program

#Question id: 12904

#SCPH28 | Zoology

Match the following:

1. Southern Blotting                               i. Melting temperature of DNA strand

2. DNA microchips                                   ii.oligonucleotide matching to DNA sample

3. ASO-PCR                                                 iii.DNA hybridization to oligo arrays

4. DGGE                                                        iv. labeled probe hybridization to DNA