Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following true about real time PCR?

TLS Online TPP Program

#Question id: 435

#SCPH06 I Botany

What defines a near-equilibrium reaction?

TLS Online TPP Program

#Question id: 10561

#SCPH05 I Biotechnology

Integral membrane proteins that form water-selective channels across the membrane, Because water diffuses faster through such channels than through a lipid bilayer

TLS Online TPP Program

#Question id: 14820

#SCPH06 I Botany

The smallest organism is probably determined by

TLS Online TPP Program

#Question id: 19377

#SCPH01 Biochemistry

Glyphosate is a broad spectrum herbicide that inhibits the enzyme

TLS Online TPP Program

#Question id: 5529

#SCPH28 | Zoology

In humans, organogenesis in the embryo is basically accomplished by the end of which period of the pregnancy?