Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

How primer concentration leads to decrease in PCR efficiency?

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#Question id: 70

#SCPH06 I Botany

The dimensions of living cells are limited, on the lower end by the minimum number of biomolecules necessary for function, and on the upper end by the rate of diffusion of solutes such as oxygen. Except for highly elongated cells, they usually have lengths and diameters in the range of

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#Question id: 11002

#SCPH28 | Zoology

A 30-year-old man has an ejection fraction of 0.25 and an end systolic volume of 150 ml. What is his end diastolic volume?

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#Question id: 4322

#SCPH01 Biochemistry

If a particular operon encodes enzymes for making an essential amino acid and is regulated like the trp operon, then

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#Question id: 5589

#SCPH01 Biochemistry

The outer coating of a pollen grain or spore is called as

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#Question id: 16772

#SCPH06 I Botany

What is the Median of the following data sample? 2, 7, 4, 8, 9, 10, 6, 12, 13