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TLS Online TPP Program

#Question id: 13038

Please arrange the following steps about ELIZA (Enzyme-linked immunosorbent assay) in chronological order
i. incubate with antibody-enzyme complex that binds primary antibody
ii. coat surface with antigen, block unoccupied sites with nonspecific protein
iii. add substrate, formation of colored product indicates presence of specific antigen
iv. incubate with primary antibody against specific antigen

#SCPH05 I Biotechnology
  1. i, iv, ii, iii
  2. i, iv, iii, ii
  3. ii, iv, iii, i
  4. ii, iv, i, iii
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TLS Online TPP Program

#Question id: 11137

#SCPH28 | Zoology

The motor (somatic nervous) system can alter the activities of its targets, the skeletal muscle fibers, because ________.
TLS Online TPP Program

#Question id: 3469

#SCPH06 I Botany

Individual A is cousin of B individual? During altruistic act A individuals get benefitted from B individual is 80. What is at least value of cost scarified by individuals A?
TLS Online TPP Program

#Question id: 13035

#SCPH01 Biochemistry

Each band on the gel represents a different protein (or protein subunit); _____ proteins move through the gel more _____ than_____ proteins and therefore are found nearer the _____ of the gel.
TLS Online TPP Program

#Question id: 15147

#SCPH12 I Genetics

You have isolated a set of five yeast mutants that form dark red colonies instead of the usual white colonies of wild-type yeast. You cross each of the mutants to a wild-type haploid strain and obtain the results shown below;
you cross each haploid mutant strain to a different haploid mutant of the opposite mating type. From the results shown below deduce as much as you can about which mutations lie in the same gene. Clearly state any remaining ambiguities and suggest some general ways that the ambiguities might be resolved
a) That mutants 1 and 3 form one complementation group and are mutations in the same gene (gene A) 
b) That mutations 2 and 5 form a second complementation group and are mutations in a second gene (gene B).
c) That mutations 3 and 5 form a second complementation group and are mutations in a second gene (gene B).
d) The first ambiguity is whether mutant 4 has a mutation in gene A or B, or whether it represents a unique gene.
Which of the following is the correct prediction about mutants?
TLS Online TPP Program

#Question id: 14271

#SCPH05 I Biotechnology

Penicillin is produced by P. chrysogenum in a fed-batch culture with the intermittent addition of glucose solution to the culture medium. The initial culture volume at quasi-steady state is V0 = 500 l, and glucose-containing nutrient solution is added with a flow rate of F = 50 l/h. Glucose concentration in the feed solution and initial cell concentration are S0 = 300 g/l and X0 = 20 g/l, respectively. The kinetic and yield coefficients of the organism are mm = 0.2 h-1 , KS = 0.5 g/l, and YX/S = 0.3 g dw/g glucose. Determine the concentration of cells at quasi-steady state when t = 10 h.