Barbara McClintock, famous for discovering that genes could move within genomes, had her meticulous work ignored for nearly 4 decades, but eventually won the Nobel Prize. Why was her work so distrusted?

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#Question id: 771

#SCPH05 I Biotechnology

MicroRNAs are:

P. synthesized as long pri-miRNAs;

Q. transcribed by RNA polymerase II;

R. synthesized from pre-microRNAs by an RNase III enzyme Drosha in the cytoplasm;

S. synthesized from pri-miRNAs by an enzyme Dicer in the nucleus.

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#Question id: 4802

#SCPH28 | Zoology

Why is tetrad analysis useful for studying genetics?

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#Question id: 1695

#SCPH01 Biochemistry

The processed peptide binding to the MHC class I groove:

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#Question id: 15142

#I Life Science/ Life Sciences Group – I-V

Dot matrix analysis of the amino acid sequences of lambda phage cI (horizontal sequence) and phage P22 c2 (vertical sequence) repressors is shown below. Which one of the following is correct?

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#Question id: 13088

#SCPH28 | Zoology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

What is the Colony 1’s plasmid is;