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TLS Online TPP Program
#Question id: 22955
#SCPH06 I Botany
Based On ABC model during flower development, loss of class A activity results the formation of only stamen and carpel. Which of the flowering floral organs identity genes controls the class A activity ?
TLS Online TPP Program
#Question id: 15705
#SCPH06 I Botany
IAA or IBA auxins alone or in combination with a low concentration of cytokinin are important in the induction of
TLS Online TPP Program
#Question id: 10517
#SCPH05 I Biotechnology
plant–insect herbivore interactions releases three types of hormone including—
TLS Online TPP Program
#Question id: 13089
#SCPH28 | Zoology
To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.
The distance between each tick mark placed on the line above is 100 bases in length
Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’
The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.
You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.
What is the Colony 2’s plasmid is;
TLS Online TPP Program
#Question id: 920
#SCPH05 I Biotechnology
Which of these is able to cross the inner mitochondrial membrane?