Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

In blue white screening insertional activation of Beta-D-galactosidase will give rise to

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#Question id: 14966

#I Life Science/ Life Sciences Group – I-V

The bacterial cell wall is composed of a macromolecular network called peplidoglycan (also known as murein) which is present either alone or in combination with other substances

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#Question id: 14966

#SCPH05 I Biotechnology

The bacterial cell wall is composed of a macromolecular network called peplidoglycan (also known as murein) which is present either alone or in combination with other substances

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#Question id: 15006

#SCPH12 I Genetics

Which of the following is not a mechanism for sex determination?

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#Question id: 15007

#SCPH12 I Genetics

You set up a cross between true breeding wild type male fruit flies and true breeding female flies with a yellow body color. You notice in the F1 generation that half the flies, specifically all the males, have yellow body color while the females are wild type (gray). Which of the following explanations is the most likely?

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#Question id: 15008

#SCPH12 I Genetics

You have a white eyed, male fruit fly with vestigial wings and a wild type female known to be heterozygous for both traits. You know that vestigial is located on an autosome. What is the probability that a mating between these two flies will generate a female offspring with vestigial wings and white eyes?

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#Question id: 15009

#SCPH01 Biochemistry

A mutation in which most of the protein coding sequence of the gene is removed is most likely to be which type of allele?